Mô tả
- Hệ thống để bàn có thông lượng cao, số lượng mẫu cao
- Mô-đun: Tối đa 48 flow cell, mỗi flow cell có tới 3.000 kênh nanopore (tổng cộng lên tới 144.000)
- Các flow cell có thể được chạy riêng lẻ hoặc đồng thời
- Quy trình làm việc tương tự như MinION ở quy mô lớn hơn
https://nanoporetech.com/products/sequence/promethion-24-48
- High-throughput, high-sample number benchtop system
- Modular: Up to 48 flow cells, each with up to 3,000 nanopore channels (total up to 144,000)
- Flow cells may be run individually or concurrently
- Same workflow as MinION at larger scale
https://nanoporetech.com/products/sequence/promethion-24-48
Publications:
Scalable Nanopore sequencing of human genomes provides a comprehensive view of haplotype-resolved variation and methylation
https://www.nature.com/articles/s41592-023-01993-x
Targeted haplotyping in pharmacogenomics using Oxford Nanopore Technologies’ adaptive sampling
https://www.frontiersin.org/articles/10.3389/fphar.2023.1286764/full
An intronic copy number variation in Syntaxin 17 determines speed of greying and melanoma incidence in Grey horses
https://www.biorxiv.org/content/10.1101/2023.11.05.565619v1
Metagenomic sequencing detects human respiratory and enteric viruses in air samples collected from congregate settings
https://www.medrxiv.org/content/10.1101/2023.05.28.23290648v2
The landscape of genomic structural variation in Indigenous Australians
https://www.biorxiv.org/content/10.1101/2023.10.17.562810v1
Two long read-based genome assembly and annotation of polyploidy woody plants, Hibiscus syriacus L. using PacBio and Nanopore platforms
https://www.nature.com/articles/s41597-023-02631-z#Sec2
Landscape of Large-Scale Somatic Genomic Insertions in Non-Small Cell Lung Carcinoma Revealed by Nanopore Sequencing
https://www.biorxiv.org/content/10.1101/2023.10.07.561149v1
Direct measurement of engineered cancer mutations and their transcriptional phenotypes in single cells
https://www.nature.com/articles/s41587-023-01949-8
Genome-wide methylation patterns from canine nanopore assemblies
https://academic.oup.com/g3journal/article/13/11/jkad203/7264045?login=false
scNanoHi-C: a single-cell long-read concatemer sequencing method to reveal high-order chromatin structures within individual cells
https://www.nature.com/articles/s41592-023-01978-w
The complete sequence of a human Y chromosome
https://www.nature.com/articles/s41586-023-06457-y#citeas
Using deep long-read RNAseq in Alzheimer’s disease brain to assess clinical relevance of RNA isoform diversity
https://www.biorxiv.org/content/10.1101/2023.08.06.552162v1
Borg extrachromosomal elements of methane-oxidizing archaea have conserved and expressed genetic repertoires
https://www.biorxiv.org/content/10.1101/2023.08.01.549754v1
Conserving a threatened North American walnut: a chromosome-scale reference genome for butternut (Juglans cinerea)
https://www.biorxiv.org/content/10.1101/2023.05.12.539246v3
Single-cell third-generation sequencing-based multi-omics uncovers gene expression changes governed by ecDNA and structural variants in cancer cells
https://onlinelibrary.wiley.com/doi/full/10.1002/ctm2.1351
Single-cell long-read targeted sequencing reveals transcriptional variation in ovarian cancer
https://www.biorxiv.org/content/10.1101/2023.07.17.549422v1
High throughput single cell long-read sequencing analyses of same-cell genotypes and phenotypes in human tumors
https://bcevietnam.com.vn/wp-admin/post.php?post=5257&action=edit
Long-read sequencing shows complex structural variants in tumor-stage mycosis fungoides
https://www.biorxiv.org/content/10.1101/2023.07.03.547529v1
JTK: targeted diploid genome assembler
https://academic.oup.com/bioinformatics/article/39/7/btad398/7206882?login=false
